Kinetics and mechanism of bioreduction of nitrimidazoles as hypoxia probes

2-Nitroimidazole derivatives have been traditionally used as molecular markers of hypoxia due to their preferential reduction and subsequent trapping in vivo under hypoxic conditions. However, the reduction mechanism and its rate determining steps remained largely unexplored. We show that it is the intracellular redox state (NADP/NADPH, GSSG/GSH), rather than the oxygen tension by itself, what determines the reduction rate of these compounds, the reaction rate being limited mainly by the GSH concentration. This is a general mechanism occurring in all 2-Nitroimidazole derivatives investigated. Also, we report the appearance and the kinetics of new signals belonging to reaction intermediates.

Trabajo presentado al 21th Annual Meeting & Exhibition of International Society for Magnetic Resonance in Medicine, celebrado en Salt Lake City (US) del 20 al 26 de Abril de 2013.

Peer Reviewed

Country

Spain

Related Organizations

Spanish National Research Council
Spain

Impact byBIP!

	selected citations These citations are derived from selected sources. This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	0
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Average
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Average
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Average