Nucleolin Is Required for DNA Methylation State and the Expression of rRNA Gene Variants in Arabidopsis thaliana

descriptionPublicationkeyboard_double_arrow_right Article 24 Nov 2010 France English Publisher:Public Library of Science (PLoS)Journal:PLoS Genetics, volume 6, page e1001225 (eissn: 1553-7404,

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Authors: Pontvianne, Frédéric; Abou-Ellail, Mohamed; Douet, Julien; Comella, Pascale; Matia, Isabel; Chandrasekhara, Chinmayi; Debures, Anne; +6 Authors

doi: 10.1371/journal.pgen.1001225

pmid: 21124873

pmc: PMC2991258

handle: 10261/103932

Nucleolin Is Required for DNA Methylation State and the Expression of rRNA Gene Variants in Arabidopsis thaliana

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Abstract

In eukaryotes, 45S rRNA genes are arranged in tandem arrays in copy numbers ranging from several hundred to several thousand in plants. Although it is clear that not all copies are transcribed under normal growth conditions, the molecular basis controlling the expression of specific sets of rRNA genes remains unclear. Here, we report four major rRNA gene variants in Arabidopsis thaliana. Interestingly, while transcription of one of these rRNA variants is induced, the others are either repressed or remain unaltered in A. thaliana plants with a disrupted nucleolin-like protein gene (Atnuc-L1). Remarkably, the most highly represented rRNA gene variant, which is inactive in WT plants, is reactivated in Atnuc-L1 mutants. We show that accumulated pre-rRNAs originate from RNA Pol I transcription and are processed accurately. Moreover, we show that disruption of the AtNUC-L1 gene induces loss of symmetrical DNA methylation without affecting histone epigenetic marks at rRNA genes. Collectively, these data reveal a novel mechanism for rRNA gene transcriptional regulation in which the nucleolin protein plays a major role in controlling active and repressed rRNA gene variants in Arabidopsis.

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France

Related Organizations

DePaul University
United States
Inserm
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French Institute of Health and Medical Research
France
University of Clermont Auvergne
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Indiana University Bloomington
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Keywords

Transcription, Genetic, [SDV.BBM.BS] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], Arabidopsis, [SDV.GEN] Life Sciences [q-bio]/Genetics, QH426-470, [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Histones, Gene Expression Regulation, Plant, RNA Polymerase I, [SDV.GEN.GPL] Life Sciences [q-bio]/Genetics/Plants genetics, [SDV.BID.SPT] Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy, DNA, Ribosomal Spacer, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.BID.EVO] Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE], Genetics, Nucleolus Organizer Region, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BC] Life Sciences [q-bio]/Cellular Biology, [INFO.INFO-BI] Computer Science [cs]/Bioinformatics [q-bio.QM], Repetitive Sequences, Nucleic Acid, [SDV.BIBS] Life Sciences [q-bio]/Quantitative Methods [q-bio.QM], Arabidopsis Proteins, Gene Expression Profiling, RNA-Binding Proteins, Genes, rRNA, DNA Methylation, Phosphoproteins, Nucleosomes, [SDV.BBM.MN] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular Networks [q-bio.MN], RNA, Plant, RNA, Ribosomal, Mutation, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BV.AP] Life Sciences [q-bio]/Vegetal Biology/Plant breeding, Protein Processing, Post-Translational, Research Article, Protein Binding

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