The aims of this study were to compare two new methods (Dmax and CUSUM) for determination of the ventilatory threshold and to examine the consequences of estimation by application of these methods in combination.a comparative design was used.the study was performed in the Exercise Physiology Laboratory in the Faculty of Medicine, Selçuk University.thirty-two untrained males (20.6 +/- 1.2 yrs) performed an incremental exercise test on a cycle ergometer.there is no intervention.ventilatory and gas exchange variables were measured breath-by-breath. The ventilatory thresholds were detected by conventional linear regression, CUSUM, Dmax and combined CUSUM-Dmax methods.The ventilatory thresholds determined by Dmax method gave the highest r-values compared to the criterion method. There was no statistical difference between thresholds determined by all methods or by the same method using different variables. Ventilatory thresholds could not be determined by the conventional linear regression method in three subjects but were determined in all subjects by the other three methods.Although all methods presented in this study can be used in the determination of ventilatory threshold, the Dmax method was found to be the most valid one. When using the CUSUM method, combining it with the Dmax method increases the validity of the measurement.