Ctenophore colloblast adhesive remains an understudied source of potential inspiration for biomedical adhesives. Adhesive is stored within small, specialised cells termed colloblasts, lining the tentacles and tentacle side branches of many ctenophore species. We use Pleurobrachia pileus as a model organism to study both the in vivo use of colloblasts and their adhesive in prey capture. We present evidence that colloblasts act as distinctive and disposable units of prey capture through observation of mechanical probing events. We add evidence to support the idea that colloblast adhesive release is mediated by membrane potential of tentacular cells, with evidence for intracellular calcium influx, potentially modulated by chemical signals representative of natural prey animals. We determine a novel method of collecting the ctenophore colloblast adhesive using high calcium artificial sea water, allowing us to create a low-cost relative adhesivity assay based on binding to the particulate dye Berlin blue. After validating this assay, we apply it to films of adhesive to show that the adhesive retains adhesivity after storage at sub-zero temperatures for at least a year, and that it degrades in adhesive ability significantly at human body temperature and above over 24 hours. We show the adhesive has a globular morphology when plated, and shows limited adhesive force under atomic force microscopy. We also show the adhesive is capable of being sterilised by UV light and ethanol while retaining some adhesive ability. We find that the adhesive contains protein, and that this protein can be separated into multiple constituents of different molecular masses via gel electrophoresis. We subject these proteins to mass spectrometry and present a group of potential protein identities based on known protein sequences from other organisms. We attempt to characterise the interaction between the adhesive and human cells using biochemical LDH and MTT assays, findings no impacts on laminal cribrosa cells, limited impacts on human dermal fibroblasts, and significant toxicity with HeLa cells. We investigate the impacts on HeLa cells further by measuring cell adherence to well substrates via Xcelligence real-time cell analysis, revealing potential impacts of the adhesive on cell proliferation. We build a full microscope for use within a tissue culture incubator to assess the impact of the adhesive on cell morphology and proliferation, and find that the adhesive triggers the HeLa cells to undergo reduced proliferation but increased cell volume, potentially representing impacts on the cell cycle of HeLa cells. We present the ctenophore colloblast adhesive as a novel biomaterial with potential biomedical applications that is worthy of further exploration.