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Anatomia vegetal aplicada ao estudo de sistemas androgênicos in vitro

Authors: Rodrigues, Lia Rosane; Oliveira, João Marcelo Santos de; Mariath, Jorge Ernesto de Araujo;

Anatomia vegetal aplicada ao estudo de sistemas androgênicos in vitro

Abstract

(Anatomia Vegetal Aplicada ao Estudo de Sistemas Androgênicos In Vitro) – Por meio do cultivo in vitro de anteras ou de gametófitos isolados de algumas espécies vegetais, é possível interromper o desenvolvimento microgametofítico e acionar divisões celulares atípicas, as quais podem originar esporófitos com a mesma constituição genética do gametófito e com variados níveis de ploidia, caracterizando a ocorrência da androgênese. Estudos histológicos têm contribuído para a compreensão dos eventos morfogênicos nesta transição direta gametófitoesporófito, que, freqüentemente, envolve a embriogênese. Além disso, diante da possibilidade de proliferação dos estratos parietais e do conectivo, o estudo histológico de anteras em cultivo tornou-se indispensável para a identificação dos sítios de embriogênese. Neste texto, são apresentadas observações recentes, resultantes de estudos histológicos em ambos os sistemas que visam à androgênese, indicando que, comparativamente a outras técnicas, as técnicas de análise anatômica oferecem inferências de grande confiabilidade.

(Applied Plant Anatomy to Study In Vitro Androgenic Systems) – By means of anther culture or isolated gametophyte culture, it is possible to control the microgametophytic development and to trigger atypical cell divisions that could originate sporophytes. These new sporophytes have the same genetic constitution of the gametophyte, with different ploidy levels, characterizing the occurrence of androgenesis. In this direct gametophyte-sporophyte transition, histological studies contribute to understanding morphogenic events, which frequently occur by embryogenesis. Moreover, since anther walls and connective tissue could proliferate in vitro, cultured anthers must always undergo histological studies for a precise description of embryogenic sites. In this text, recent histological observations in both androgenic systems are presented and discussed. Observations confirm that compared with techniques, anatomical techniques enable highly reliable inferences.

Keywords

Histology, Embriology, Androgênese, Embryogenesis, Plant anatomyembriology, Androgenesis, Anatomia vegetal, Histologia vegetal, Embriogenese

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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