
handle: 10174/38231
O presente estudo analisou dados relativos à exsudação e teor de colagénio total em músculos peitorais de frango com as miopatias Wooden Breast e White Striping, colhendo os seguintes grupos de amostras: músculos sem lesões, com lesões intermédias e com lesões graves. Procedeu-se à determinação do teor de exsudado pelo método EZ-Driploss e pelo método pistométrico, e à determinação do teor de colagénio total, com recurso ao kit da Sigma- Aldrich (Referência: MAK463) e protocolo de determinação espectrofotométrica do conteúdo de hidroxiprolina. Os dados recolhidos mostram que existe uma correlação positiva entre a gravidade da lesão e a exsudação. O kit MAK463 não permitiu a recolha de dados. Porém, com o protocolo referido, verificou-se uma correlação positiva entre o teor de colagénio total e a gravidade da lesão. Pode concluir-se, que a presença de lesão aumenta, de forma acentuada e progressiva, a exsudação e a deposição de colagénio intramuscular; - Abstract: Myopathies in Broiler chickens The present study analysed data about exudation and total collagen content in the pectoral muscles of broilers with Wooden Breast and White Striping myopathies, collecting the groups of samples: muscles without lesion, with moderate lesion and with severe lesion. The exudate content was determined with the EZ-Driploss method, and the pistometric method, and the total collagen content was determined using a kit from Sigma-Aldrich (Reference: MAK463) and a spectrophotometric determination protocol for hydroxyproline content. The collected data shows that there is a positive correlation between the severity of the lesion and exudation. The kit MAK463 did not allow collection data. However, with the spectrophotometric protocol, there is a positive correlation between the total collagen content and the severity of the lesion. It can be concluded that the presence of lesion increases, markedly and progressively, the exudation and intramuscular collagen deposition.
Frango, Colagénio, Myopathy, Broiler, Exudation, Miopatia, Collagen, Músculo Peitoral, Exsudação, Pectoral Muscle
Frango, Colagénio, Myopathy, Broiler, Exudation, Miopatia, Collagen, Músculo Peitoral, Exsudação, Pectoral Muscle
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