Demodex folliculorum and Demodex brevis mites are parasites of humans only. These mites are usually found on face, eyebrows, eyelashes and meibomian glands. When they appear in large numbers in eyelashe follicules, they may cause Demodex blepharitis. The aim of this study was to determine the prevalence of Demodex species on eyelashes of healthy individuals. Eyelashes were collected from 131 healty individuals who did not have a history of blepharitis. While D. folliculorum was detected in 17 (13.0%) people, D. brevis was only seen in four individuals (3.1%), the differences being statistically significant. It was observed that D. folliculorum occurs more commonly in older people and male/female rates were not different. Since the D. brevis was rarely found in eyelashes, cases of blepharitis are believed to be the result of the co-existance of D. brevis and D. folliculorum.