This study was carried out in order to investigate the presence of Neospora spp. in horse in the Bor, Ulukisla, Camardi and Altunhisar districts from Nigde province. This is the first serologic survey for neosporosis performed on horses in Nigde province. Serum samples were obtained from a total of 125 horses (50 male, 75 female) which were 1-20 years old and were tested for antibodies to Neospora spp. using the Enzyme Linked Immunosorbent Assay (ELISA). In this study, chi-square test was used to compare the seropositivity rates in terms of sex, age groups and study districts. According to the serological examination, the 30 (24%) out of 125 horses were seropositive for Neospora spp. Anti-Neospora spp. antibodies were detected in 14 (28%) of 50 male horses and 16 (21.3%) of 75 female horses. There was no statistically significant difference in seropositivity between genders (P=0.393). Antibodies to Neospora spp. were found in 81(22.2%) horses at the age of 1-10 and 44(27.2%) at the age of 11-20. A statistically insignificant difference between the age groups was observed for antibodies Neospora spp. (P=0.528). Besides, the seropositivity rates were detected 40%, 17.1%, 20% ye 25.7% in the Bor, Ulukisla, Camardi and Altunhisar from the Nigde, respectively. The difference between seropositivity rates from the selected districts in Nigde was statistically indifferent (P=0.254).