Dentin hypersensitivity (DH) is a relatively common painful condition among dental problems for which several treatment options have been tested. Although many studies have been performed regarding the diagnosis and treatment of DH, dental professionals are still confused about the definite diagnosis and treatment. The neurosensory mechanisms for dentin hypersensitivity are still unclear. According to the hydrodynamic theory, DHS is related to fluid movements within exposed dentinal tubules. Although, this theory cannot explain for all pain associated with DHS. TRPC5 also known as transient receptor protein 5 (TRP-5) is a protein that in humans is encoded by the TRPC5 gene. In this chapter we will study the role of odontoblasts when equipped with the cold-sensor TRPC5 along with TRPA1, can lead to sensitivity.