Acacia mearnsii is characterized as a tree with dark green leaves and grow on any types of soil. Its bipinnate leaves with individual leaflets that are used in folk medicine in wound healing, antioxidant, anti-inflammatory and antiviral. Phytochemical tests demonstrated the presence of saponins, flavonoids and tannins. The haemolytic activity evaluation of dry crude buthanolic leaves extract of A. mearnsii confirming the presence of saponins as a chemical marker of this species presenting a low haemolytic content in comparison with Tribullus terrestres, herbal reference product.