Investigating transgenic characteristics of Californium, Jura, Elvis and Orkan varieties of canola hasbeen carried out in this study. These plants are widely cultivated in Turkey. The Canola varieties weregrown in an established tissue cultu re and the total DNA isolated.The 35S and pNOS promoter region of the transgene were scanned using classic and multiplex PCR techniques. The transfer of transgenic characteristic to the NAD gene region confirms the accuracy of the PCR technique. This study, has determined that Californium, Jura, Elvis and Orkan varieties of canola consist of transgenic characteristics and all the four varieties have multiple insertion region in terms of CaMV 35S and Pnos promoters.