Asaia spp. has recently been reported to cause opportunistic infections in humans and is becoming an emerging hospital pathogen. To our knowledge, this is the first report on Asaia spp. in Malaysia. bacteremia in an infant. A girl with underlying Hirschsprung's disease, who was on parenteral feeding via a central venous catheter, developed persistent multidrug-resistant Gram-negative bacteremia. Routine automated identification methods failed to identify the organism, which was later identified by 16S ribosomal RNA sequencing. Bacterial clearance was achieved after the removal of the catheter and initiation of IV amikacin. This case highlights the role of molecular identification and the clinical importance of Asaia spp. in causing infections in humans, especially in patients with indwelling devices.