Platelets as one of the key indicators in blood tests, reflect the number of platelets in the blood, thereby aiding doctors in assessing the blood's coagulation and hemostatic functions. Abnormal platelet counts may indicate a variety of health issues, including hemorrhagic diseases, thrombotic diseases, anemia, leukemia, and more. Therefore, ensuring the accuracy of platelet test results is crucial for the diagnosis and treatment of diseases.This research report describes two cases of pseudothrombocytopenia. Given that large platelets and EDTA-dependent platelet aggregation may interfere with the test results, we adopted the flow cytometry platelet (PLT-F) mode and re-sampling with a different anticoagulant to correct the interference in the sample.After excluding interfering factors, the platelet counts returned to normal levels, consistent with the patient's clinical condition. Therefore, the two cases of abnormally low platelet counts were identified as pseudo-reductions caused by interfering factors.For samples with abnormally low platelet counts, the laboratory should first identify potential interfering factors and take appropriate measures to correct these interferences to ensure accurate and error-free test results for doctors and patients.