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In vitro Tissue Culture of Brassica oleracea var Italica Curd

Authors: ALMA B. SEGISMUNDO; MAY EVELIA V. RUADAP;

In vitro Tissue Culture of Brassica oleracea var Italica Curd

Abstract

This experimental research determined the feasibility of micropropagating in vitro,broccoli from its curd through plant tissue culture technology to be able to produceplants that are free from any disease. The growth of broccoli (Brassica oleraceavar Italica curd in vitro was measured in terms of organ formation and the cultures’average height (cm) after the observation period. It also determined the percentsurvivorship of the plant cultures after the observation period. The method usedwas adopted from Balcita (2011) utilizing the modified Murashige and Skoog (MS)medium. Result of the study show that broccoli plantlets grew from the curd after asix-week observation period. Addition of thiamine in the culture medium protectedthe cultures from bacterial or fungal infections, thus coming up with very healthyplantlets and a high survivorship of the cultures was also observed. In conclusion,propagation of broccoli curd in vitro is feasible using the modified Murashige andSkoog (MS) medium; plantlets with roots, stem, leaves, and curd with florets can grow directly from the broccoli curd; and there is a high percent survivorship in thecultures with the addition of thiamine in the culture medium.

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
gold