The Cold Seeps Extreme24 Expedition was the second leg of two expeditions, which form part of the University of Tromsø’s Extreme 24 project. The expedition focused on cold seeps sites characterised by methane emissions in several localities in the Barents Sea. The expedition is a research initiative (PI G. Panieri) hosted by the Department of Geosciences, part of the Faculty of Science and Technology at UiT, the Arctic University of Norway, situated in Tromsø, and involved partners from different research institutes and universities around the world and used the REV Ocean Remotely Operated Vehicle (ROV) Aurora. The expedition has been developed by leveraging the extensive knowledge gained from two significant projects funded by the Norwegian Research Council: CAGE (Centre for Arctic Gas Hydrate, Environment and Climate) and AKMA (Advancing Knowledge of Methane in the Arctic), both hosted at the Department of Geosciences at UiT. These projects have provided fundamental understandings to define the objectives and methodologies used during the EXTREME24 expedition, aiming to broaden our understanding of Arctic extreme environments.