We used adipocyte-derived stem cells in combination with previously used methods (platelet-rich plasma and cell seeding) to create a 3D bioprint of skin on a hydrophobic matrix dressing. The patient was a 16-year-old girl with 65% total body surface area burns who had had multiple previous operations, and required additional skin with minimal donor sites. The patient had a Pseudomonas aeruginosa wound infection that compromised graft take. The overall graft take at 2 weeks was between 20 and 60%. The results were satisfactory in this patient, but there is plenty of room for improvement to achieve 100% graft take. Further technical refinements in the technique and postoperative management could improve results.