Background: Angiopoietin-like protein 4 (ANGPTL4) has been demonstrated highly expressed in some cancers, but it also was downregulated in others through DNA methylation. However, the expression status of ANGPTL4 in cancer of cervix remains unclear. Thus, our present study attempts to investigate the role of ANGPTL4 in the prognosis of patients with cervical cancer. Methods: Immunohistochemistry, western blotting and qRT-PCR were performed to investigate the expressive level of ANPGTL4 in cervical cancer tissues and paired noncancer tissues. The relationship of ANGPTL4 expression and clinicopathological factors was measured by Chi-square test. Overall and disease-free survival (OS and DFS) rates were estimated and compared by using Kaplan-Meier method and log-rank test, respectively. To assess the prognostic significance of ANGPTL4 in cervical cancer patients, the Cox regression model was utilized to process univariate and multivariate analyses. Results: ANGPTL4 was upregulated in cervical cancer samples and advanced tumor stage, deep stromal invasion, lymph node metastasis, lymphovascular space invasion, as well as poor OS and DFS were shown to be tightly correlated with it. Results of Cox multivariate analysis demonstrated that ANGPTL4 expression was an independent prognostic factor for OS and DFS in cervical cancer. Conclusion: Upregulated ANGPTL4 seems to be a useful marker for poor prognosis in cervical cancer.