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Biopolymers and Cell
Article . 2010 . Peer-reviewed
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Biopolymers and Cell
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Наукова електронна бібліотека періодичних видань НАН України (Vernadsky National Library of Ukraine)
Article . 2019
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Genome variability of some Gentiana L. species in nature and in culture in vitro: RAPD-analysis

Геномна мінливість деяких видів роду Gentiana L. у природі та в культурі in vitro: RAPD-аналіз
descriptionPublicationkeyboard_double_arrow_right Article 20 Nov 2010Publisher:Institute of Molecular Biology and Genetics (NAS Ukraine)Journal:Biopolymers and Cell, volume 26, pages 499-507 (issn: 0233-7657, eissn: 1993-6842, Copyright policy )
Authors: Twardovska, M.O.; Drobyk, N.M.; Mel'nyk, V.M.; Konvalyuk, I.I.; Kunakh, V.A.;

doi: 10.7124/bc.00017a

Genome variability of some Gentiana L. species in nature and in culture in vitro: RAPD-analysis

Abstract

????????. ?????????????????????? ?????????????????????????? ?? ???????????????????????????? ???????????????????????? Gentiana acaulis, G. cruciata ?? G. punctata. ????????????. ???????????????????????? ???????????? ?????????????? ?? ???????????????????? ???????????????????????? ???????????????????????????????????? (RAPD-??????), ????????-????????????????????????. ????????????????????. ???????????????????? ?????????????????????????????????? ?????????????????????? ???????????????????????? ???????????????????????????? ?????????????????? ??? ???????????????? ?????????????????????? ???????????????????? ?? ???????????????????????? ????????????????????. ?????????????? ?????????????????????????? ???????????????????????? ?????????????????????? ?? ???????? G. acaulis > G. punctata > G. cruciata. ????????????????, ?????? ?????????????????? ?? ???????????????? ???????????? ?????????????????? ???? ???????????????????? ?????????????????????? ???????????????????? ?????????? ?? ?????????????????? 10??? 15 % ?? ???? ?????????????? ???? ?????????????? ?????????????????????????? ????????????????????????????. ????????????. ?????????????? RAPD-?????? ?????????????????????? ???????????????? ???????????????????????? G. acaulis, G. cruciata ?? G. punctata. ??????????????????????, ?????? ?????????????????? ?????????????????????????????? ?????????????????? ?????????????????????????????? ?????? ?? ??????????????, ?????? ?? ?? ???????????????? in vitro. ?? ???????????????? ???????????? ???????????????????? ?????????????????????? ?????????????? ?????????????????? ???? ?????????????????? ?? ?????????????????????????? ??????????????????????????.

????????. ?????????????????? ???????????????????????????????? ???? ?????????????????????????? ???????????????????? Gentiana acaulis, G. cruciata ???? G. punctata. ????????????. ?????????????????????? ?????????????????? ?????????????? ?? ???????????????????? ?????????????????? ?????????????????????????? (RAPD- ??????), ????????-????????????????????????. ????????????????????. ???????????????? ?????????????????????????????????? ???????????????????? ???????????????????? ???????????????????????????? ???????????????? ??? ???????????????? ?????????????????????? ???????????????????? ?? ???????????????????? ??????????????????. ???????????? ?????????????????????????????????? ???????????????????? ?????????????????????? ?? ???????????????? G. acaulis > G. punctata > G. cruciata. ????????????????, ???? ?????????? ?? ???????????????? ???????????? ???????????????? ???? ?????????????? ?????????????????????? ???????????????????? ???????????? ?? ?????????????????? 10??? 15 % ?? ???? ???????????????? ???? ???????? ?????????????????????????????????? ????????????????????????????. ????????????????. ?????????????? RAPD-?????? ???????????????????? ?????????????? ???????????????????? G. acaulis, G. cruciata ???? G. punctata. ??????????????????????, ???? ?????????????? ???????????????????????????????? ???????????? ???????????????????????????? ???? ?? ??????????????, ?????? ?? ?? ???????????????? in vitro. ?? ???????????????? ???????????? ???????????????? ??????????, ?????? ???????????? ?????????? ?????????????????? ?? ?????????????????????????????????? ????????????????????.

Aim. Investigation of intraspecies and somaclonal variability of G. acaulis, G. cruciata and G. punctata. Methods. Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR), gel-electrophoresis. Results. It was established a species specificity for indicators of gentians genetic heterogeneity ??? the proportion of polymorphic amplicons and genetic distances. A level of intraspecies variation decreased in the direction of G. acaulis > G. punctata > G. cruciata. Changes in gentians tissue culture by the proportion of polymorphic amplicons were shown to vary within the range of 10???15 % and failed to extend beyond the intraspecies variation. Conclusions. Genome variation of G. acaulis, G. cruciata and G. punctata was studied through the use of RAPD-PCR. The gentians were found to be characterized by different variability in both nature and culture in vitro. In the tissue culture there were discovered considerably smaller number changes as compared to intraspecies variation.

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Keywords

Genomics, Transcriptomics and Proteomics

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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