Bocashi is a fermented organic fertilizer widely used in sustainable agriculture due to its ability to improve soil fertility and provide essential nutrients for plant development. In the Ecuadorian Amazon region, where soils are often acidic and nutrient-poor, this type of amendment represents a viable alternative to chemical fertilizers. The objective of this study was to characterize the content of macronutrients (nitrogen, phosphorus, potassium, calcium, and magnesium) and micronutrients (zinc and iron) in three formulations of bocashi produced at the Universidad Estatal Amazónica. Compost piles measuring 12 m × 1 m × 0.6 m were constructed and fermented for 14 days with daily turning. Samples were analyzed at the Environmental Studies Laboratory using validated techniques such as atomic absorption spectrophotometry, the Kjeldahl method, and colorimetry. The results showed a slightly alkaline pH (8.08–8.15), with adequate levels of nitrogen (0.56–0.62%), phosphorus (33.8–35.2 ppm), potassium (7110–7192 mg/kg), calcium (29203–30463 mg/kg), magnesium (2019–2039 mg/kg), zinc (335.9–393.3 mg/kg), and iron (835.8–1383 mg/kg). These values reflect the agronomic quality of locally produced bocashi and its potential to enhance nutrient availability in tropical soils, promoting sustainable agricultural practices in the Ecuadorian Amazon.