Floral nectar is the primary reward offered to pollinators by plants and therefore it is an important determinant in plant-pollinator interactions. Global warming and land use changes have significantly reduced pollinator numbers. Understanding plant-pollinator ecology is imperative to maintain these populations. Pollination syndromes are plants with specialised floral traits evolved to attract specific pollinators. This study investigates if nectar sugar concentration varies across bat, bird, and insect pollination syndromes at La Selva Biological Station, Costa Rica. This study aims to better understand if pollination syndromes influence floral nectar sugar concentration. We found a significant relationship between pollination syndrome and nectar sugar concentration (p < 0.005) with chiropterophilous flowers having the lowest sugar concentration. We conclude that pollination syndromes have a significant effect on floral nectar sugar concentrations in the taxa studied at La Selva. However existing research reveals that this relationship is not observed across all species and locations. Other factors like phylogeny and pollinator specificity can have a greater effect on nectar sugar concentration than pollination syndrome. Further research should examine how phylogeny and pollinator specificity, alongside pollination syndrome, influence nectar sugar concentration in plants at La Selva.