Background: A search for novel anxiolytic agents has been the prime target for neuroscientists as the currently available treatment methods have certain limitations like physiological dependence, delayed efficacy and side effects. However, naturally occurring agents are raising hopes. The present study was aimed to evaluate the effects of Ziziphus jujube honey over stress related anxiety behavior in Swiss Albino mice. Methods: Following Jujube honey administration (at 2g/kg, 4g/kg and 6g/kg; body weight), the experimental mice were employed in open field and hole-cross test apparatus to evaluate neurologic properties and the anxiolytic activities were investigated using elevated plus maze test (EPM). Mice behavior were observed through parameters of square crossing, rearing, grooming, hole crossing, entry and duration in open arm at different time intervals. Results: At higher doses (6g/kg), Jujube honey was found to possess mild sedative-anxiolytic activity whereas in lower doses (2g/kg & 4g/kg), a non-sedative; anxiolytic potential was observed. Diazepam was effective enough to reduce the locomotor activity and anxiety in all the experiments specially at late phases. Conclusion: The results demonstrated that the anti-anxiety potential of Jujube honey is dose dependent and could be utilized in broad spectrum in inducing sedation and reducing anxiety in mice. Therefore, jujube honey potentiates scopes for further research as a CNS agent.