The batrachofaunistic studies of the City of Trebinje, situated in the southern part of the Republic of Srpska (SE Bosnia and Herzegovina) used to be rather neglected. The goal of this paper was to create a faunistic list of amphibians in the City of Trebinje, according to both original field research and literature references, with an overview of certain aspects of ecology and conservation status of the recorded species. The review of literature references, combined with six years of field research by the authors, has shown that batrachofauna of the City of Trebinje includes eight species of amphibians (Proteus anguinus, Lissotriton graecus, Bufo bufo, Bufotes viridis, Bombina variegata, Hyla arborea, Pelophylax ridibundus and Rana graeca), systematized into eight genera, six families and two orders. Three species (Bombina variegata, Hyla arborea and Rana graeca) are recorded for the study area for the first time. Four of the recorded taxa are endemic for Balkans/Dinarides. This paper also includes certain ecological data (which are scarce in literature pertaining to the study area) and a comparative overview of the conservation status of the recorded species.