Smoking is one of the leading preventable causes of disease and death worldwide and is classified as a chronic disease due to nicotine dependence. In this context, pharmacists have proven to be essential professionals in smoking cessation efforts, working directly in primary care and public health programs. This study aimed to assess the degree of nicotine dependence among students at the Federal University of Mato Grosso (UFMT) – Sinop Campus, using the Fagerström Test, and to discuss the pharmacist’s role in this context. This is an investigative study approved by the Research Ethics Committee (Opinion No. 4.471.865/2020). The target population consisted of 136 students over 18 years of age, randomly selected. Data were collected through an electronic form containing demographic information and the Fagerström Test. The data were organized into tables and graphs and analyzed using Microsoft Excel. Most participants (70.6%) were between 18 and 24 years old. It was observed that 71.3% did not use any type of cigarette, and among smokers, 19.1% used electronic cigarettes. Nicotine dependence was classified as low to moderate in most cases, with only 2.9% of participants reporting smoking within the first five minutes after waking up — an indicator of high dependence. The analysis also showed that 1.5% reported difficulty giving up the first cigarette of the day, while 46.2% reported difficulty giving up any cigarette, suggesting a consolidated habit. The study demonstrated that the application of the Fagerström Test is a useful tool for identifying the degree of nicotine dependence, contributing to the development of effective intervention strategies. The pharmacist’s role is fundamental both in administering the test and in providing clinical, educational, and motivational support to patients. Their involvement in smoking cessation through counseling, pharmacotherapeutic follow-up, and participation in health campaigns is essential for the success of treatment and prevention of relapse, promoting significant improvements in quality of life and public health.