Objective: This study aims to optimize the method of simple paraffin section, and provide reference for the study of histopathological techniques. Methods: We comprehensively considered and controlled the environmental humidity, paraffin quality and soaking time to improve the quality of paraffin sections. Only in this way can high-quality paraffin sections be obtained, providing more accurate results for tissue structure observation and analysis. Results: Achieving high-quality paraffin sections crucial for accurate tissue structure analysis relies on meticulous control of environmental humidity, consistent paraffin quality, and optimized soaking time. Maintaining appropriate indoor humidity levels (25℃-30℃) ensures optimal paraffin solidification and slicing, while utilizing high-quality paraffin enhances section quality. Additionally, adjusting soaking time (8-12h) based on tissue characteristics is essential for optimal results. By addressing these key factors, researchers can obtain superior paraffin sections, facilitating precise tissue structure observation and analysis. Conclusions: Environmental humidity, paraffin quality, and soaking time are essential for producing high-quality paraffin sections, which are imperative for accurate analysis of tissue structure.