Figure S1. Genotyping methods applied to confirm probiotic origin of clinical yeast samples. a. Karyotyping results. b. Mitochondrial DNA RFLP with HaeIII. c. Mitochondrial DNA RFLP with MboI. d. Mitochondrial COX1 intron typing. e. Microsatellite-primed PCR with (GTG)5 primer. f. Interdelta typing with primers δ1-2. g. Interdelta typing with primers δ12-2. h. Microsatellite typing for YPL009C. i. Microsatellite typing for YGLO13C. j. Microsatellite typing for YLR177W.Figure S2. Multiplex PCR method to identify probiotic-borne Saccharomyces infections. Comparison of different DNA sources and enzymes. Gel electrophoresis in 2% TBE agarose, 100V, 1.5 h. Lanes 1-7: GoTaq polymerase (Thermo Fisher Scientific) with genomic DNA isolated with glass-bead method. 8-14: GoTaq polymerase with colony DNA. 15-21: DreamTaq (Promega) with genomic DNA isolated with glass-bead method. Strains and isolates: 1: Probiotic yeast manufactured by Biocodex, batch I. 2: Probiotic yeast manufactured by Biocodex, batch II. 3: Probiotic yeast manufactured by Wotania, batch I. 4: Probiotic yeast manufactured by Wotania, batch II. 5: Isolate DE27020, from bronchus, non-mycosis. 6: Isolate DE6507, from haemoculture, fungaemia. 7: isolate DE35762, from haemoculture, fungaemia. M: 1 kb Plus Size ladder (Thermo Fisher Scientific).