Our current understanding of respiratory physiology and pathophysiological mechanisms of lung diseases is often limited by challenges in developingin vitromodels faithful to the respiratory environment, both in cellular structure and physiological function. The recent establishment and adaptation of microfluidic-basedin vitrodevices (μFIVDs) of lung airways have enabled a wide range of developments in modern respiratory physiology. In this paper, we address recent efforts over the past decade aimed at advancingin vitromodels of lung structure and airways using microfluidic technology and discuss their applications. We specifically focus onμFIVDs covering four major areas of respiratory physiology, namely, artificial lungs (AL), the air-liquid interface (ALI), liquid plugs and cellular injury, and the alveolar-capillary barrier (ACB).