Currently there are several culture systems that can provide disease-free mini tubers, and provide multiple yield of seed potato as compared to the conventional methods. The minituberization process was evaluated on different culture systems, in a bifactorial experience, type 4*4, where factor a was culture system, with four gradations: a1 - system without automation on industrial substrate; a2 - nutrient system film technique NFT; a3 - Wilma system; a4 - conventional system, without automation on peat-perlite substrate, and factor b: variety, with four graduations: Azaria, Brașovia, Cosiana and Cezarina. Culture system, variety and their interaction significantly influenced number and weight of potato mini tubers. The values of number and weight of mini tubers obtained in the system without automation on perlite substrate differ significantly, in a positive sense, from the other systems. The variety Azaria recorded the highest average number of mini tubers in the 4 culture systems, followed by the variety Cezarina. The NFT culture system contributed significantly to increasing minituberization capacity for Azaria variety, which led to the highest number of mini tubers recorded.