The main goal of the root canal treatment is to completely eliminate the different components of the pulpal tissue, calcification and bacteria, and to prevent infection or re-infection . Irrigants perform both biological and physical roles during endodontic treatments. An irrigant ideally provides a mechanical flushing action and dissolve the remnants of organic tissues without damaging the periradicular tissues if extruded into the periodontium. Irrigation plays the main role in eradication of microbes from the root canal system. Instrumentation, irrigation, locally used disinfecting agents, and root filling (sealer) all contribute to the killing and removal of microbes from the infected root canal, with irrigation regarded as the most important factor.