During routine mycological survey in Satara district of Maharashtra, India. A powdery mildew infection was observed on Cucurbita maxima Duchesne. Microscopic examinations revealed the identity of powdery mildew as Fibroidium sp. During mycological examinations powdery mildew infection was found to be mixed with brownish intercellular pycnidia. These brown coloured pycnidia were picked up with the help of a needle under dissecting microspore and mounted in lactophenol and stained with cotton blue. Critical examinations identified it as a mycoparasite namely Ampelomyces quisquali Ces.