Eclipta alba, commonly known as False Daisy is an important medicinal plant and its different parts are famous for the treatment of different health problems including digestion, headache, asthma, cough and normalizing skin colour. Keeping in view the importance of E. alba, it was analyzed quantitatively, qualitatively for its phytochemicals (alkaloids, flavonoids, saponins, tannins, glycosides, terpenoids, reducing sugars, anthraquinones, and cardiacglycoside) physiochemicals, and anti-fungal activity. For anti-fungal activity, four different strains including Aspergillus niger, Aspergillus fumigatus, Fusarium solani and Aspergillus flavus used has shown very promising results against the fungal strain.