Identification and characterization of 16 recent Chinese isolates of bovine viral diarrhea virus genotype 2 (BVDV-2)
Copyright policy )Identification and characterization of 16 recent Chinese isolates of bovine viral diarrhea virus genotype 2 (BVDV-2)
Eighty calf blood samples were collected from different areas of China and were examined. Sixteen non cytopathic isolates of bovine viral diarrhea virus genotype 2 (BVDV-2) were isolated by specific BVDV-2-directed RT-PCR and sandwich ELISA. The results were verified by the indirect immunofluorescence assay and the ultra-thin sections. Genes of 5'-UTR, E2 and Npro from all the 16 field isolates were sequenced. The sequence identities at the nucleotide and amino acid levels were higher than 99.0%. The phylogenetic analysis revealed that the BVDV-2 USA strain, AzSpin, had the highest sequence homology with each of the Chinese isolates. Key words: Bovine viral diarrhea virus genotype 2 (BVDV-2), isolation and characterization, phylogenetic analysis.
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