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African Journal of Biotechnology
Article . 2009 . Peer-reviewed
Data sources: Crossref
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African Journal of Biotechnology
Article
License: CC BY
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Somatic embryogenesis in Mucuna pruriens

English
Authors: Harini, SS; Sathyanarayana, N;

Somatic embryogenesis in Mucuna pruriens

Abstract

This study reports the induction of somatic embryos in Mucuna pruriens. Different explants cultured on MS medium supplemented with 11.31 ìM 2,4-D produced golden yellow embryogenic callus that induced synchronized embryo development on MS basal liquid medium. Organization of pre-embryonic mass was noticed 15 d after sub culturing the callus, which progressively developed to globular, heart, torpedo and cotyledonary shaped embryos. Attempts to germinate them did not succeed as the cotyledonary embryos turned characteristic red and subsequently brown before finally turning blackand unresponsive. The reaction was consistent across different media constituents, hormonal concentrations and pH ranges. Supplementation of anti-oxidants was also ineffective. Polyacrylamide gel electrophoretic analysis of the brown and non-brown embryos revealed quantitative differences inprotein contents between the two. More detailed study is necessary to establish the precise cause for embryo browning and ways of its regulation.

Keywords

Mucuna, somatic embryogenesis, embryo-browning, antioxidants, L-Dopa.

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
1
Average
Average
Average
gold