The purpose of this research was to study the information seeking behavior of librarians in central libraries of state higher education institutions in terms of information seeking objective, information sources, information resources, methods and problems of information seeking, and the usage of information sources and resources received. The research results revealed that librarians in central libraries of state higher education institutions seek information for the objective of routine work usage, increase general knowledge and experience, improve work efficiency, solve problem while working, and follow-up on professional improvement in the high level. The source of information used in the high level and had the highest mean score was the central libraries that the librarians work in.The criteria in choosing the information source with the high level and had the highest mean score was information that was up to date. The high level of information resource sought by librarians was from the website. The high level and the highest mean score of information subject sought by librarians was information technology. The high level of information seeking method used by librarians was OPAC searching. The high level and the highest mean score of information seeking problem was not being able to find the titles of information sought from OPAC on the library shelf. The source of information that librarians was able to make the most usage in the high level and had the highest mean score was the central library that the librarians work in. Finally, the information resource that librarians was able to make the most usage in the high level and had the highest mean score was website. The analysis of variance is used to investigate the significant difference of information seeking behavior of acquisition librarians, catalogers and reference librarians in terms of information seeking objective, information sources, information resources, methods and problems in information seeking. The result showed that the majority had no significant difference.