Antibiotic Susceptibility Test is measurement of the susceptibility of bacteria to antibiotics. Antibiotic Susceptibility Test may be done if infection does not respond to treatment1. It helps doctor to recommend the most vital and effective Antibiotic to overcome an infecting micro-organism, pathogenic organisms such as bacteria, fungus etc. that infect our body and lodge infection. Antibiotic Susceptibility Test specifies effective Antibiotic dosage and evaluating Antibiotic Resistance and helps in determining patient’s treatment plans. This testing technique is widely used as micro-organisms tend to develop resistant to some antibiotics. It is used to select effective drugs for treatment. It determines ability of the drug to kill bacteria. It is used to check effective antibiotic or a combination of few antibiotics that are most effective in treating the different types of bacteria causing the infection, present in the diagnostic sample2. It is used to recognize causative organism and also check if it shows a demonstrable and comparable sensitivity pattern.