Recent advances in technology have accelerated the understanding of the genetic basis of phenotypes. With these developments, genomics has altered the way that biological challenges are thought about on a genome-wide (genome-wide) scale, revealing a large amount of information and creating a myriad of opportunities. One of these recently developed fields is bioinformatics, which uses the concept of computer Science, mathematics, molecular biology and statistics to store, retrieve & analyze biological data. Even though it is still in its infancy, it has quickly emerged as one of the field with the quickest growth rates and established itself as a crucial part of any biological research program. It is becoming more well-known because of its capacity to quickly and affordably analyze vast amounts of biological data. A biologist can use a variety of web- and/or computerbased tools provided by bioinformatics to extract valuable information from biological data, the majority of which are free to use. This introductory chapter aims to provide overall picture on basics and advancement in the field of bioinformatics benefitting readers in various fields of Biological science