The application of AuNPs on the infected barley cultivar had great damage results on Barley Yellow Dwarf Virus (BYDV-PAV) particles in TEM. Observation of TEM images provided an insight into the transport of AuNPs through the plasmodesmata endoplasmic reticulum route, where they likely accumulated as the channels narrowed. The cytoplasmic parenchyma cell components do not have an intact peripheral location, but taking irregular shapes, internal movement between adjacent two cells seems to be the VLPs moved toward via plasmodesmata. TEM micrographs; showing different abnormalities in the cell wall due to viral infection. Application of AuNPs revealed sticky Integrated AuNPs inside the cell wall with low and high density. The mechanical transportation of the virus through the sieve elements with endosomes was observed. The mechanical transportation of virus particles through the cell wall with some vesicles, amorphous inclusions, and filamentous particles was proved through the sieve elements with filamentous strands.