Graphene paper as a new form of graphene-supported nanomaterials has received worldwideattention since its first report in 2007. Due to their high flexibility, lightweight andgood electrical conductivity, graphene papers have demonstrated the promising potentialfor crucial applications in electrochemical sensors and energy technologies amongothers. In this chapter, we present some examples to overview recent advances in theresearch and development of two-dimensional (2D) graphene papers as new materialsfor electrochemical sensors. The chapter covers the design, fabrication, functionalizationand application evaluations of graphene papers. We first summarize the mainstreammethods for fabrication of graphene papers/membranes, with the focus on chemicalvapour deposition techniques and solution-processing assembly approaches. A largeportion of this chapter is then devoted to the highlights of specific functionalization ofgraphene papers with polymer and nanoscale functional building blocks for electrochemical-sensing purposes. In terms of electrochemical-sensing applications, the emphasis ison enzyme-graphene and nanoparticle-graphene paper-based systems for the detectionof glucose. We finally conclude this chapter with brief remarks and outlook.