Nigella sativa oils have anti-inflammatory, antibacterial, antifungal, antiparasitic and antiprotozoal, antiviral, cytotoxic, anticancer, neuro-, gastro-, cardio-, and hepatoprotective properties, making them potential treatments for a wide range of illnesses. N. sativa oil also suggests positive benefits on the immunological, respiratory, and reproductive systems in addition to diabetes mellitus (DM), fertility, breast cancer, dyspepsia, osmotic balance, and other conditions. Thymoquinone (TQ) is a suitable target for its potential antibacterial, antimicrobial, anti-inflammatory, chemopreventive, antitumoral, and other actions among the various isolated chemical moieties. The N. sativa oil has been shown in various non-clinical and clinical investigations to benefit health. On the other hand, TQ in several animal experiments is clear to generate no adverse modifications of the body biomarkers; rather, it enhanced health quality. This study presents a more mechanistic review of the constitutions and oil of N. sativa. In conclusion, research on Nigella oil may represent a health breakthrough.