Brassinosteroids (Br) have been shown to favor the growth and reproduction of crops under adverse environmental conditions, which negatively affect their growth and production. In order to solve some of the problems in the field with various perennial crops, the application of a homobrassinolide (HBr) (CIDEF-4) has been investigated under in vitro and ex vitro conditions to evaluate growth at different concentrations in Musa spp. L. and Saccharum officinarum L and in the field with foliar applications in Theobroma cacao L, Mangifera indica L and Coffea arabica L. to evaluate yield and quality of fruits. Morphological and physiological yield components were recorded. The results indicate in the in vitro evaluations, increased regrowth height and ex vitro differences in growth are improved by increasing the number of applications. In cocoa and coffee plants, flowering and yield are influenced. The high concentrations applied did not necessarily increase the crop yield or the combination with potassium nitrate. In mango, the quality of the fruits was better when applying the HBr alone or in combination with nitrate in fruit firmness, and total soluble solids improved.