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https://doi.org/10.5772/intech...
Part of book or chapter of book . 2025 . Peer-reviewed
License: CC BY
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Controlled Phase Shifting in Reflective Digital Holographic Microscopy

Authors: Diego Palacios; Michael R. Wang;

Controlled Phase Shifting in Reflective Digital Holographic Microscopy

Abstract

Digital holographic microscopy (DHM) with 3D microscopic imaging capability is attractive for biological sampling, particle identification, and surface topographic profiling. The Michelson (Linnik) optical configuration along with microscope objectives is used to create a hologram with the reflections of a sample of 3D spatial structures. Instead of instantaneous volume recording of the hologram in a holographic film, a digital camera is used to sequentially record four phase-step holograms in 2D format, namely the digital holograms (DHs). A piezoelectric actuator was added to implement the formation of the four-phase-step DHs. Using a four-image phase-shifted algorithm, the 3D microscopic image of the object can be extracted. The recording of the DHs is fast, 28 ms, and the phase retrieval computation is fast as well, 24 ms. The DHM has been utilized to examine the surface topography of a lithography sample showing field of view of 312 μm × 234 μm, axial resolution of 3.31 nm, lateral resolution of 488 nm, and a maximum topography height of 270 nm.

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
hybrid