Rift Valley fever virus (RVFV) is a negative-sense, single-stranded RNA virus in the genus Phlebovirus of the family Phenuviridae. It is an arbovirus transmitted mainly by mosquitoes. RVFV is an emerging zoonotic disease where humans can get infected via exposure of mucosa or broken skin to infected animals or through mosquito transmission. The disease was first detected in sheep in the Rift Valley region in Kenya in 1930, and since then, several outbreaks have been reported in northern and sub-Saharan Africa and Middle Eastern countries. North America is considered free of RVF, but the mosquito vectors involved in the transmission of RVF are present in many regions of the American and European continents. The virus causes disease in livestock characterized by hemorrhagic fever. The severity of the disease varies among different species, with sheep experiencing the most severe infection. Young as well as pregnant animals may also be severely affected by RVFV. For laboratory diagnosis, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is the most commonly used test. Other assays, including serological assays and nucleic acid-based point-of-care tests, are also available for RVF diagnosis. There is no specific treatment for RVFV infection. Control and preventive measures should include vaccination, vector control, and proper hygiene.