Predictive genomics can support treatment decisions by giving people the chance to act in time to prevent serious illness. Tests based on single nucleotide polymorphism (SNP) can be analyzed by various methods. Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry technology detects genetic variants based on their individual mass. Standardized workflow, automation, sensitivity, quick turnaround time, and reliability are the main advantages of the MALDI-TOF use in molecular analysis. Beside pharmacogenetics, SNP variation plays a role in various fields of medicine. In the present article importance of various SNPs for nutrigenetics is presented. Especially, various aspects of fat metabolism, vitamin metabolism, and intolerances were discussed.