Haploids are plants (sporophytes) that contain a gametic chromosome number (n). They can originate spontaneously in nature or as a result of various induction techniques. Spontaneous development of haploid plants has been known since 1922, when Blakeslee first described this phenomenon in Datura stramonium (Blakeslee et al., 1922); this was subsequently followed by similar reports in tobacco (Nicotiana tabacum), wheat (Triticum aestivum) and several other species (Forster et al., 2007). However, spontaneous occurrence is a rare event and therefore of limited practical value. The potential of haploidy for plant breeding arose in 1964 with the achievement of haploid embryo formation from in vitro culture of Datura anthers (Guha and Maheshwari, 1964, 1966), which was followed by successful in vitro haploid production in tobacco (Nitsch and Nitsch, 1969). Many attempts have been made since then, resulting in published protocols for over 250 plant species belonging to almost all families of the plant kingdom (reviewed in Maluszynski et al., 2003). In fact, under optimal conditions, doubled haploids (DH) have been routinely used in breeding for several decades, although their common use is still limited to selected species. There are several reasons for this. These might be categorized as biological, based on plant status (annual, biannual, perennial, authogamous, allogamous, vegetativelly propagated) and flower morphology or technical, which are the result of the feasibility and efficiency of DH induction protocol. Induction protocols substantially vary, in fact, not only among species but also among genotypes of the same species.