Musashi1 (Msi1) is an evolutionary conserved RNA-binding protein that plays important roles in neural stem cell maintenance, nervous system development, and tumorigenesis. In glioblastoma, Msi1 is found to be highly expressed and to control a network of cancerrelated genes. In this chapter, we will review the participation of RNA-binding proteins in tumorigenesis and the role of Msi1 in stem cells and in glioblastoma. Furthermore, we will discuss the results of a study done with The Cancer Genome Atlas (TCGA) dataset to map genes highly correlated in expression with Msi1 as an avenue to understand its function in gliomagenesis.