The incomplete information on most reported macrofungi is one of the remaining challenges of taxonomic work in Indonesia. Cookeina tricholoma (Ascomycetes, Pezizales, Sarcoscyphaceae), otherwise known as cup fungi, is no exception. In this study, we report C. tricholoma from West Java, Indonesia. Fresh materials were evaluated using morphological and molecular analyses. The specimen was illustrated with macro- and microscopic features. Molecular analyses were performed using the internal transcribed spacer region. Cookeina voucher BO24423 was identified as C. tricholoma. The morphological characteristics of C. tricholoma BO24423 are cup-shaped, pinkish to reddish, and with prominent hairs. The phylogenetic tree analyses confirmed that our specimens were C. tricholoma with 99–100% bootstrap value. This study is the first contribution to the identification of C. tricholoma based on morphological and molecular evidence for Indonesia.