Introduction: BK virus (BKV) is highly prevalent in the general population, in renal transplant (RT) patients it is the main cause of infectious tubulointerstitial nephritis, leading to graft dysfunction or loss. The seroprevalence in the Mexican population is unknown. The present study seeks to establish the seroprevalence of VBK in renal donors and recipients in the Mexican adult population. Methods: This is a cross-sectional study conducted at the Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán in Mexico City in the period June 2017, April 2018. Seroprevalence prior to renal transplantation was evaluated through qualitative BK-IgG ELISA (MyBioSource Inc, San Diego, CA), in donors and recipients of RT. Results: Qualitative measurement of IgG for BK virus by ELISA method was performed in 80 donors and 88 renal recipients. The seroprevalence in donors was 56.3% (95%CI, 45%-67%), while in recipients it was 45.5% (95%CI, 35%-56%). None of the variables analyzed showed an association with increased risk of seropositivity for BKV in either recipients or donors. Conclusion: The seroprevalence of BKV in our center was lower than expected for a developing country, having a sufficient sample size to extrapolate it to the Mexican population.