Sarcocystis spp. is a prevalent parasite found in livestock raising significant concerns for public health due to the potential risks associated with the consumption of undercooked or raw meat. Sarcocystis species have been identified in various animals worldwide yet our study marks the first investigation of Sarcocystis infection in slaughtered sheep in and around Hyderabad, Telangana. The prevalence of ovine sarcocystosis was investigated by immuno fluorescent antibody technique standardized in our laboratory. Purified bradyzoites collected from microcysts of Sarcocystis spp. were used to prepare antigenic slides. The sera sample collected from a sheep prior to slaughter whose muscle sample were found positive for the bradyzoites of Sarcocystis spp. by Pepsin HCl acid muscle digestion was used as known positive serum. A blood sample from a newborn lamb was used as a negative reference serum in all the tests. The prepared antigen slides reacted well with positive serum emitting bright yellowish-green fluorescence whereas negative serum with reddish fluorescence or polar fluorescence or no fluorescence. The seroprevalence of ovine sarcocystosis, determined by the laboratory-standardized IFAT, was 58% (145 out of 250) of tested sheep sera. The prevalence varied with age, with a lower infection rate (47%) observed in the 1-2 years age group and an increasing rate (65.3%) in those older than two years. Significant difference (p<0.05) in infection was noticed between sheep older than 2 years and 1-2 year. No significant difference (p>0.05) in infection was noticed between male (56%) and female (59.3%) animals.