A micropropagation protocol of Artocarpus heterophyllus Lamk (Moraceae) was successfully established. Apical shoots from a 12-year- old A. heterophyllus tree were used as explants. The shoot explants that were cultured on Murashige and Skoog (MS) (1962) medium supplemented with 4.5 mg/L BA produced the most number of shoots (seven shoots per explant) after three to four weeks of culture. The in vitro shoots cultured on half strength MS medium supplemented with 0.5 mg/L NAA + 0.5 mg /L IBA and placed in total darkness for 10 days followed by light exposure for three weeks produced long, normal and healthy roots. About 70-80 per cent of the in vitro A. heterophyllus plantlets survived after acclimatisation and transferred to plant house. Keywords: Acclimatisation, apical shoots, Artocarpus heterophyllus Lamk, micropropagation, multiple shoots, plantlets