The genus Tilia (Tiliaceae) represents 45 species, of which six species are European. Tilia sp. are simple, cordate or deciduous trees with long, silicate shaped, 5-valve fruits and fragrant flowers. It is used as a medicinal tea in traditional medicine for colds, coughs, and hypertension and as an antioxidant. Within the scope of this research, Tilia platyphyllos Scop. in the culture form from Eskişehir was obtained, and infusions were prepared at different times (including 5 min., 10 min., 15 min., and 30 min., respectively). Headspace-solid-phase microextraction (HS-SPME) combined with gas chromatography-mass spectrometry (GC-MS) was used to examine the volatile components in the infusion extracts. Major volatile compounds of infusion extracts determined as E)-β-ocimene (15.7-45.9%) and limonene (11.7-33.4%), respectively. Also, terpinolene (26.0%) were identified as the main compounds for 30 min infusion. After then, the infusion extracts were lyophilized, and the antioxidant activity of the infusion extracts were performed by DPPH· radical scavenging effect. It was determined that inhibition percentages were relatively high in the concentration range of 10-0.02 mg/mL (5.60-72.45%). Our first research was the chemical composition and biological activity of the time-dependent T. platyphyllos infusions.