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Article . 2010 . Peer-reviewed
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Extração e caracterização de uma enzima proteolítica do curauá (Ananas Erectifolius)

Authors: Ferreira, J.f.; Sbruzzi, D.; Barros, K.v.g.; Tambourgi, E.b.; Machado, Isaac Stringueta;

Extração e caracterização de uma enzima proteolítica do curauá (Ananas Erectifolius)

Abstract

O curauá (Ananas erectifolius) é uma planta fibrosa encontrada no Norte e Centro-Oeste do Brasil, uma bromeliácea de características físico-químicas que lhe conferem grande potencial de utilização na indústria automobilística, como fonte de fibras. Como toda planta da família Bromeliaceae contém níveis significativos da enzima bromelina, de alto valor comercial e com ampla aplicação também na indústria farmacêutica, alimentícia e cosmética. Neste trabalho, foram realizados testes experimentais de extração da enzima proteolítica do curauá sob diferentes condições de pH e temperatura, medindo-se a atividade enzimática para cada um dos ensaios, utilizando-se o reativo de Biureto e determinando-se a temperatura e pH ótimos de sua utilização, ou seja, valores em que a atividade enzimática da enzima é máxima, com o objetivo de otimizar as condições de uso do resíduo para posterior purificação. Foram utilizadas as duas variedades comercialmente encontradas (branca e roxa). Os resultados mostraram que o curauá possui enzimas com atividade proteolítica, sendo o pH ótimo 8,5 para as duas variedades e temperaturas ótimas de 30 ºC para a espécie branca, e 10 °C, 20 °C e 35 ºC, para a roxa.

Country
Brazil
Keywords

Atividade Enzimática, pH, Ananas erectifolius L.B.Smith, caracterização enzimática, Characterization, Enzime, Temperature, Temperatura, Bromelina, Enzymatic, Activity, Ananas erectifolius, Bromelain

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
1
Average
Average
Average
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